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Human Collagen IV alpha3 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27405

Novus Biologicals, part of Bio-Techne
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NBP3-27405

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.113 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Collagen IV alpha3 ELISA Kit (Colorimetric)

ELISA: Human Collagen IV alpha3 ELISA Kit (Colorimetric) [NBP3-27405] -

Human Collagen IV alpha3 ELISA Kit (Colorimetric) - Standard Curve Reference

Kit Contents for Human Collagen IV alpha3 ELISA Kit (Colorimetric)

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Collagen IV alpha3

COL4A3 (Collagen, type IV, alpha 3) belongs to the type IV collagen family. Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Type IV collagen is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity.

Alternate Names

alpha-3 polypeptide, collagen, type VI, alpha 3, DKFZp686D23123, FLJ98399

Gene Symbol

COL4A3

Additional Collagen IV alpha3 Products

Product Documents for Human Collagen IV alpha3 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Collagen IV alpha3 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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