Human Collagen Type I ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30102
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (example only; lot dependent) (100 uL (example only; lot dependent))
Sensitivity
0.059 ng/mL (example only; lot dependent)
Assay Range
0.156-10 ng/mL (example only; lot dependent)
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Specificity
The positive control is from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. The human based material used in this product is screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.
Assay Procedure Summary
1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37C.
8. Add 50ul Stop Solution. Read at 450 nm immediately.
Assay Procedure Summary
1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37C.
8. Add 50ul Stop Solution. Read at 450 nm immediately.
Description
Collagen Type I (COL1) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL1 in human serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)
Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)
Scientific Data Images for Human Collagen Type I ELISA Kit (Colorimetric)
ELISA: Human Collagen Type I ELISA Kit (Colorimetric) [NBP2-30102]
Kit Contents for Human Collagen Type I ELISA Kit (Colorimetric)
- Assay Diluent A - 12 mL
- Assay Diluent B - 12 mL
- Detection Reagent A - 120 uL
- Detection Reagent B - 120 uL
- Positive Control (lyophilized)
- Precoated microtiter plate - 1EA
- Standard - 2 vials
- Standard Diluent - 20 mL
- Stop Solution - 6 mL
- TMB Substrate - 9 mL
- Wash Buffer (30X) - 20 mL
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage is content dependent.
Background: Collagen Type I
Alternate Names
Collagen I, Collagen Type 1
Additional Collagen Type I Products
Product Documents for Human Collagen Type I ELISA Kit (Colorimetric)
Product Specific Notices for Human Collagen Type I ELISA Kit (Colorimetric)
The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20C. After reconstitution, store Positive Control vial at 4C and use within 5 days.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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