Human Complement C4b ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18769

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56-100 ng/mL

View all Complement C4b ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Complement C4b in samples. No significant cross-reactivity or interference between Human Complement C4b and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.65

Inter-Assay Precision (Precision between assays) %CV < 5.34

Recovery for Human Complement C4b ELISA Kit (Colorimetric)

Recovery

83-108%

Linearity

Human Complement C4b ELISA Kit (Colorimetric)

Scientific Data Images for Human Complement C4b ELISA Kit (Colorimetric)

Human Complement C4b ELISA Kit (Colorimetric)

ELISA: Human Complement C4b ELISA Kit (Colorimetric) [NBP3-18769] -

ELISA: Human Complement C4b ELISA Kit (Colorimetric) [NBP3-18769] - Standard Curve Reference

Kit Contents for Human Complement C4b ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Complement C4b

C4b encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.

Alternate Names

basic C4, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3, C4B1, C4B12, C4B2, C4B3, C4FMGC164979, CH, Chido form of C4, CO4C4B5, complement C4-B, complement C4B1a, complement component 4B, complement component 4B (Chido blood group), CPAMD3FLJ60561, EC 2.1.1.144, EC 2.7.11

Gene Symbol

C4B

Additional Complement C4b Products

Product Documents for Human Complement C4b ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Complement C4b ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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