Corticosterone Parameter Assay Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # KGE009
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (10 µL)
Sensitivity
0.047 ng/mL
Assay Range
0.103-25 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Corticosterone Parameter Assay Kit
The Parameter Corticosterone assay is a 3.5 hour competitive enzyme immunoassay designed to measure Corticosterone in cell culture supernates, serum, plasma, and urine.
Product Specifications
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Reactivity
Multi-Species
Specificity
Corticosterone in human, mouse, and rat samples.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.24 | 3.98 | 8.05 | 1.39 | 4.08 | 8.21 |
| Standard Deviation | 0.078 | 0.179 | 0.605 | 0.099 | 0.241 | 0.462 |
| CV% | 6.3 | 4.5 | 7.5 | 7.1 | 5.9 | 5.6 |
Recovery for Corticosterone Parameter Assay Kit
The recovery of Corticosterone spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 114 | 107-124 |
| Human EDTA Plasma (n=4) | 108 | 87-127 |
| Human Heparin Plasma (n=4) | 107 | 97-122 |
| Human Serum (n=4) | 110 | 92-130 |
| Mouse EDTA Plasma (n=4) | 104 | 96-111 |
| Mouse Serum (n=4) | 96 | 79-113 |
| Rat EDTA Plasma (n=4) | 98 | 77-114 |
| Rat Serum (n=4) | 99 | 87-108 |
| Urine (n=4) | 105 | 92-121 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Corticosterone were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Corticosterone Parameter Assay Kit
Multi-species Corticosterone ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Corticosterone
Alternate Names
17-Deoxycortisol;C21H30O4
Additional Corticosterone Products
Product Documents for Corticosterone Parameter Assay Kit
Product Specific Notices for Corticosterone Parameter Assay Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.