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Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06884

Novus Biologicals, part of Bio-Techne
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NBP3-06884

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

Product Specifications

Assay Type

Competitive-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Corticotropin Releasing Factor in samples. No significant cross-reactivity or interference between Rat Corticotropin Releasing Factor and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 6.06

Inter-Assay Precision (Precision between assays) 5.89

Recovery for Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric)

Recovery

84-99 %

Linearity

Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric)

Scientific Data Images for Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric)

Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric)

ELISA: Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric) [NBP3-06884] -

ELISA: Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric) [NBP3-06884] - Standard Curve Reference

Kit Contents for Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Corticotropin Releasing Factor

Corticotropin-releasing hormone (CRH) is a 41-amino acid peptide derived from a 191-amino acid preprohormone. CRH issecreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in CRH hasbeen observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin dificiency hasmultiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to productionin the hypothalamus, CRH is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed inthe placenta. In the placenta CRH is a marker that determines the length of gestation and the timing of parturitionand delivery. A rapid increase in circulating levels of CRH occurs at the onset of parturition, suggesting that, inaddition to its metabolic functions, CRH may act as a trigger for parturition. (provided by RefSeq)

Alternate Names

corticoliberin, corticotropin releasing hormone, Corticotropin-releasing hormone, CRFCorticotropin-releasing factor

Gene Symbol

CRH

Additional Corticotropin Releasing Factor Products

Product Documents for Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Corticotropin Releasing Factor ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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