Human Cortisol ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1885

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Saliva (50 uL)

Sensitivity

0.3 ng/mL

Assay Range

0.1 to 30 ng/mL

View all Cortisol ELISA Kits »

Product Specifications

Assay Type

Quantification

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Cortisol ELISA Kit is an enzyme immunoassay for the quantitative determination of Cortisol in human saliva.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 8.27%

Inter-Assay Precision (Precision between assays) CV% < 8.33%

Kit Contents for Human Cortisol ELISA Kit (Colorimetric)

  • Control 1 & Control 2: For control values and ranges please refer to vial label; ready to use.
  • Enzyme conjugate: Cortisol conjugated to horseradish peroxidase; ready to use.
  • Microtiterwells: 12x8 (break apart) strips; wells coated with an anti-cortisol antibody (polyclonal).
  • Standard A (Standard 0)* , 0 ng/mL, ready to use.
  • Standard B (Standard 1)* , 0.1 ng/mL, ready to use.
  • Standard C (Standard 2)* , 0.4 ng/mL, ready to use.
  • Standard D (Standard 3)* , 1.7 ng/mL, ready to use.
  • Standard E (Standard 4)* , 7 ng/mL, ready to use.
  • Standard F (Standard 5)* , 30 ng/mL, ready to use.
  • Stop Solution: Contains 2 N acidic solution; ready to use. Avoid contact with Stop Solution. It may cause skin irritations and burns.
  • Substrate Solution: Contains Tetramethylbenzidine (TMB); ready to use.
  • Wash Solution (10x concentrated)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cortisol

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Alternate Names

Compound F, Hydrocortisone

Additional Cortisol Products

Product Documents for Human Cortisol ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Cortisol ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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