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Mouse Creatine kinase MT 1A ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75315

Novus Biologicals, part of Bio-Techne
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NBP2-75315

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.94 mIU/mL

Assay Range

1.56 - 100 mIU/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse CKMT1A in samples. No significant cross-reactivity or interference between Mouse CKMT1A and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.97%

Inter-Assay Precision (Precision between assays) CV% < 5.16%

Recovery for Mouse Creatine kinase MT 1A ELISA Kit (Colorimetric)

Recovery

86-101%

Linearity

Mouse Creatine kinase MT 1A ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Creatine kinase MT 1A ELISA Kit (Colorimetric)

Mouse Creatine kinase MT 1A ELISA Kit (Colorimetric)

ELISA: Mouse Creatine kinase MT 1A ELISA Kit (Colorimetric) [NBP2-75315] -

ELISA: Mouse Creatine kinase MT 1A ELISA Kit (Colorimetric) [NBP2-75315] - Standard Curve Reference

Kit Contents for Mouse Creatine kinase MT 1A ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Creatine kinase MT 1A

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to thecytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes,sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two differentoligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes.Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; thismay be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrialcreatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes locatednear each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.(provided by RefSeq)

Alternate Names

Acidic-Type Mitochondrial Creatine Kinase, CKMT, CKMT1, CKMT1A, Creatine Kinase U-Type, Mitochondrial, Creatine Kinase, Mitochondrial 1 (Ubiquitous), Creatine Kinase, Mitochondrial 1A, EC 2.7.3.2, Mia-CK, Ubiquitous Mitochondrial Creatine Kinase, U-MtCK

Gene Symbol

CKMT1A

Additional Creatine kinase MT 1A Products

Product Documents for Mouse Creatine kinase MT 1A ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Creatine kinase MT 1A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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