Rat Creatine kinase MT 1B ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75318

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

1.69 ng/mL

Assay Range

2.81 - 180 ng/mL

View all Creatine kinase MT 1B ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CKMT1B in samples. No significant cross-reactivity or interference between Rat CKMT1B and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.26%

Inter-Assay Precision (Precision between assays) CV% < 4.43%

Recovery for Rat Creatine kinase MT 1B ELISA Kit (Colorimetric)

Recovery

89-106%

Linearity

Rat Creatine kinase MT 1B ELISA Kit (Colorimetric)

Scientific Data Images for Rat Creatine kinase MT 1B ELISA Kit (Colorimetric)

Rat Creatine kinase MT 1B ELISA Kit (Colorimetric)

ELISA: Rat Creatine kinase MT 1B ELISA Kit (Colorimetric) [NBP2-75318] -

ELISA: Rat Creatine kinase MT 1B ELISA Kit (Colorimetric) [NBP2-75318] - Standard Curve Reference

Kit Contents for Rat Creatine kinase MT 1B ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Creatine kinase MT 1B

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq]

Alternate Names

Acidic-Type Mitochondrial Creatine Kinase, CKMT, CKMT1, CKMT1B, Creatine Kinase U-Type, Mitochondrial, Creatine Kinase, Mitochondrial 1 (Ubiquitous), Creatine Kinase, Mitochondrial 1B, EC 2.7.3.2, Mia-CK, Ubiquitous Mitochondrial Creatine Kinase, UMTCK, U-MtCK

Gene Symbol

CKMT1B

Additional Creatine kinase MT 1B Products

Product Documents for Rat Creatine kinase MT 1B ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rat Creatine kinase MT 1B ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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