Human CRTAC1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24606

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

1.17 ng/mL

Assay Range

4.68 ng/mL - 300 ng/mL

View all CRTAC1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human CRTAC1. No significant cross-reactivity or interference between human CRTAC1 and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8.00

Inter-Assay Precision (Precision between assays) %CV < 10.00

Recovery for Human CRTAC1 ELISA Kit (Colorimetric)

Recovery

86 - 107%

Scientific Data Images for Human CRTAC1 ELISA Kit (Colorimetric)

Human CRTAC1 ELISA Kit (Colorimetric)

Human CRTAC1 ELISA Kit (Colorimetric) [NBP3-24606] -

Human CRTAC1 ELISA Kit (Colorimetric) [NBP3-24606] - Standard Curve Reference

Kit Contents for Human CRTAC1 ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CRTAC1

CRTAC1 (cartilage acidic protein 1; also CEP-68) is a 95-105 kDa member of a novel family of EGF domain-containing proteins. It is secreted by articular chondrocytes and may play a role in either cartilage matrix organization, or cell-matrix adhesion. Mature human CRTAC1 is 634 amino acids (aa) in length. It contains four FG-GAP (PheGly-GlyAlaPro) domains (aa 46-437) and one EGF-like motif (aa 559-605). Multiple splice forms exist. There are two alternate start sites at Met9 and Met211 that may be accompanied by a 39 aa substitution for the C-terminal 55 aa, or an 84 aa substitution for aa 545-661. Over aa 28-661, human CRTAC1 shares 91% aa identity with mouse CRTAC1. This form in mouse, however, is more equivalent to the human isoform that shows a C-terminal 39 aa substitution. In this case, there is 95% aa identity between mouse and human.

Long Name

Cartilage Acidic Protein 1

Alternate Names

ASPIC1, CEP68

Gene Symbol

CRTAC1

Additional CRTAC1 Products

Product Documents for Human CRTAC1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human CRTAC1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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