Rat Cyclin A1 ELISA Kit (Colorimetric)

Name: Rat Cyclin A1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-75070

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75070

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all Cyclin A1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CCNA1 in samples. No significant cross-reactivity or interference between Rat CCNA1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.55%

Inter-Assay Precision (Precision between assays) CV% < 4.88%

Recovery for Rat Cyclin A1 ELISA Kit (Colorimetric)

Recovery

87-107%

Linearity

Scientific Data Images for Rat Cyclin A1 ELISA Kit (Colorimetric)

ELISA: Rat Cyclin A1 ELISA Kit (Colorimetric) [NBP2-75070] -

ELISA: Rat Cyclin A1 ELISA Kit (Colorimetric) [NBP2-75070] - Standard Curve Reference

Kit Contents for Rat Cyclin A1 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Cyclin A1

The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34.

Alternate Names

CCNA1

Gene Symbol