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Human DEFA6 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24605

Novus Biologicals, part of Bio-Techne
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NBP3-24605

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

0.039 ng/mL

Assay Range

0.156 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human DEFA6. No significant cross-reactivity or interference between human DEFA6 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8.00

Inter-Assay Precision (Precision between assays) %CV < 10.00

Recovery for Human DEFA6 ELISA Kit (Colorimetric)

Recovery

81 - 100%

Scientific Data Images for Human DEFA6 ELISA Kit (Colorimetric)

Human DEFA6 ELISA Kit (Colorimetric)

Human DEFA6 ELISA Kit (Colorimetric) [NBP3-24605] -

Human DEFA6 ELISA Kit (Colorimetric) [NBP3-24605] - Standard Curve Reference

Kit Contents for Human DEFA6 ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: DEFA6

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 6, is highly expressed in the secretory granules of Paneth cells of the small intestine, and likely plays a role in host defense of human bowel. [provided by RefSeq]

Alternate Names

DEF6HD-6, defensin 6, Defensin, alpha 6, defensin, alpha 6, Paneth cell-specific, defensin-6

Gene Symbol

DEFA6

Additional DEFA6 Products

Product Documents for Human DEFA6 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human DEFA6 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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