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Human Dysferlin - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-31477

Novus Biologicals, part of Bio-Techne
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NBP3-31477

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.141 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Dysferlin - Ready-To-Use ELISA Kit (Colorimetric)

Human Dysferlin - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human Dysferlin  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31477] -

ELISA: Human Dysferlin  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31477] - Standard Curve Reference

Kit Contents for Human Dysferlin - Ready-To-Use ELISA Kit (Colorimetric)

  • Detection Solution A
  • Detection Solution B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Dysferlin

Dysferlin is the protein product of the 2p13 gene that is defective in patients with Limb-Girdle Muscular Dystrophy type 2B (LGMD2B) and Miyoshi Myopathy (MM). Dysferlin is normally localized to the muscle plasma membrane. In patients with LGMD2B and MM, immunoreactivity to dysferlin is severely reduced or lost, depending on the type of mutation. This antibody is used for the characterization of LGMD2B and MM.

Alternate Names

dysferlin, dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive), dystrophy-associated fer-1-like 1, FER1L1Dystrophy-associated fer-1-like protein, Fer-1-like protein 1, FLJ00175, FLJ90168, LGMD2B

Gene Symbol

DYSF

Additional Dysferlin Products

Product Documents for Human Dysferlin - Ready-To-Use ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Dysferlin - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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