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Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80133

Novus Biologicals, part of Bio-Techne
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NBP2-80133

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, and other biological fluids. (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31-20 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human ETRB in samples. No Significant cross-reactivity or interference between Human ETRB and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.73%

Inter-Assay Precision (Precision between assays) CV% < 5.14%

Recovery for Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric)

Recovery

91-109%

Linearity

Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric)

Scientific Data Images for Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric)

Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric)

ELISA: Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric) [NBP2-80133] -

ELISA: Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric) [NBP2-80133] - Standard Curve Reference

Kit Contents for Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: EDNRB/Endothelin R Type B

The Endothelin Receptor ETB is expressed in most tissues. Mutations of the receptor gene are responsible for Hirschsprung disease type 2, a multigenic disorder with phenotypes such as bicolored irides, megacolon, hypopigmentation, and hearing loss. The ETB receptor has similar affinity for all three endothelins and activates a phosphatidylinositol-calcium second messenger system. A splice variant, termed SVR, has been described; the sequence of the ETB-SVR receptor is identical to ET-B receptor, with the exception of the intracellular C-terminal domain. While both splice variants bind endothelin ET1, they exhibit different responses, which suggests that they may be functionally distinct. The endothelin B receptor has been reported to be expressed in adrenal, aorta, artery, brain, breast, eye, epididymis, ganglion, heart, kidney, liver, lung, ovary, placenta, prostate, skin, testis, uterus, and vessel. ESTs have been isolated from amnion, B-cell/lung/testis, breast, brain, colon, ear, embryo, eye, heart, kidney, liver, liver/spleen, lung, lymph node, placenta, prostate, and skin libraries.

Long Name

Endothelin Receptor Type B

Alternate Names

EDNRB, ET-B, ETRB

Gene Symbol

EDNRB

UniProt

Additional EDNRB/Endothelin R Type B Products

Product Documents for Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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