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Human Endorepellin/Perlecan/Heparan Sulfate Proteoglycan - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-31898

Novus Biologicals, part of Bio-Techne
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NBP3-31898

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

49 pg/mL (example only; lot dependent)

Assay Range

125 - 8000 pg/mL (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images

Human Endorepellin/Perlecan/Heparan Sulfate Proteoglycan - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human Endorepellin/Perlecan/Heparan Sulfate Proteoglycan  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31898] -

ELISA: Human Endorepellin/Perlecan/Heparan Sulfate Proteoglycan  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31898] - Standard Curve Reference

Kit Contents

  • Detection Solution A
  • Detection Solution B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Endorepellin/Perlecan

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodeling (2).

Long Name

Heparan Sulfate Proteoglycan

Alternate Names

HSPG2, Perlecan

Gene Symbol

HSPG2

Additional Endorepellin/Perlecan Products

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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