Human eNOS ELISA Kit (Colorimetric)

Name: Human eNOS ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-80134

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80134

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 ul)

Sensitivity

37.50 pg/mL

Assay Range

62.50-4000 pg/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human NOS3/eNOS in samples. No Significant cross-reactivity or interference between Human NOS3/eNOS and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.47%

Inter-Assay Precision (Precision between assays) CV% < 4.6%

Recovery for Human eNOS ELISA Kit (Colorimetric)

Recovery

92-108%

Linearity

Scientific Data Images for Human eNOS ELISA Kit (Colorimetric)

ELISA: Human eNOS ELISA Kit (Colorimetric) [NBP2-80134] -

ELISA: Human eNOS ELISA Kit (Colorimetric) [NBP2-80134] - Standard Curve Reference

Kit Contents for Human eNOS ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: eNOS

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.

Long Name

Endothelial Nitric Oxide Synthase

Alternate Names

NOS3

Gene Symbol