Human Ephrin-B2 ELISA Kit (Colorimetric)

Name: Human Ephrin-B2 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-18214

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18214

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell lysates, tissue homogenates and other biological fluids (100 ul)

Sensitivity

0.078 ng/ml

Assay Range

0.156-10 ng/ml

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human Ephrin-B2 in the sample, this Human Ephrin-B2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human Ephrin-B2 concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 15

Scientific Data Images for Human Ephrin-B2 ELISA Kit (Colorimetric)

ELISA: Human Ephrin-B2 ELISA Kit (Colorimetric) [NBP3-18214]

ELISA: Human Ephrin-B2 ELISA Kit (Colorimetric) [NBP3-18214] - Standard Curve Reference

Kit Contents for Human Ephrin-B2 ELISA Kit (Colorimetric)

Balance Solution
Enzyme Conjugate
Microtiter Plate
Standards
Stop Solution
Substrate A
Substrate B
Wash Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Ephrin-B2

Ephrins are naturally divided into two structural groups. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins, which can be tyrosine phosphorylated upon receptor ligation. Class B ephrins show 33% amino acid sequence identity in their extracellular segments and 44% amino acid sequence identity in their cytoplasmic regions.

Alternate Names

EFNB2, ELF-2, EphrinB2, Htk-L, LERK-5, NLERK-1

Gene Symbol

EFNB2

Additional Ephrin-B2 Products

Product Documents for Human Ephrin-B2 ELISA Kit (Colorimetric)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Manual - Human Ephrin-B2 ELISA Kit (Colorimetric)

COA

SDS

Download SDS

Product Specific Notices for Human Ephrin-B2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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