Human F-Spondin/SPON1 ELISA Kit (Colorimetric)

Name: Human F-Spondin/SPON1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-40108

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-40108

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.212 ng/mL (example only; lot dependent)

Assay Range

0.625 - 40 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human F-Spondin/SPON1 ELISA Kit (Colorimetric)

ELISA: Human F-Spondin/SPON1 ELISA Kit (Colorimetric) [NBP3-40108]

ELISA: Human F-Spondin/SPON1 ELISA Kit (Colorimetric) [NBP3-40108] - Standard Curve Reference

Kit Contents for Human F-Spondin/SPON1 ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: F-Spondin/SPON1

SPON1 is a member of a subgroup of the thrombospondin type 1 (TSR) class molecules, defined by two domains of homology, the FS1/FS2 and TSR domains. The TSRs of SPON1 proteins are typical of class 2 TSRs. SPON1, which is similar to thrombospondin, is a extracellular matrix attached molecule that promotes neurite outgrowth and inhibits angiogenesis. Analysis of gain and loss of function experiments reveal that SPON1 is required for accurate pathfinding of embryonic axons, and plays a dual role in patterning axonal trajectories. It promotes the outgrowth of commissural and inhibits the outgrowth of motor axons, and has also been implicated in inflammatory processes in the nervous system.

Alternate Names

FSpondin, SPON1, VSGP

Gene Symbol

SPON1

Additional F-Spondin/SPON1 Products

Product Documents for Human F-Spondin/SPON1 ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human F-Spondin/SPON1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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