Human Factor V ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82505

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

37.5 pg/mL

Assay Range

62.5 - 4000 pg/mL

View all Coagulation Factor V ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Factor V in samples. No Significant cross-reactivity or interference between Human Factor V and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.43

Inter-Assay Precision (Precision between assays) CV% < 5.19

Recovery for Human Factor V ELISA Kit (Colorimetric)

Recovery

87-106%

Linearity

Human Factor V ELISA Kit (Colorimetric)

Scientific Data Images for Human Factor V ELISA Kit (Colorimetric)

Human Factor V ELISA Kit (Colorimetric)

ELISA: Human Factor V ELISA Kit (Colorimetric) [NBP2-82505] -

ELISA: Human Factor V ELISA Kit (Colorimetric) [NBP2-82505] - Standard Curve Reference

Kit Contents for Human Factor V ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Coagulation Factor V

The F5 gene encodes a 2,224 amino acid long, 251 kDA coagulation factor V protein that is critical in the regulation of homeostasis. Additionally, F5 functions as a cofactor for the prothrombinase activity of factor Xa which results in the initiation of prothrombin to thrombin. F5 participates in blood coagulation signaling pathways, the blood clotting cascade, platelet degranulation as well as activation, signaling, and aggregation, and in responses to elevated platelet cytosolic Ca2+. It interacts with genes PROC, PROS1, F2, MMRN1, and CALR. Defects in F5 cause factor 5 deficiency (owren parahemophilia), thrombophilia due to activated protein C resistance, susceptibility to Budd-Chiari syndrome, susceptibility to ischemic strokes, and susceptibility to pregnancy loss, recurrent, type 1. F5 is also linked to deep vein thrombosis, antithrombin III deficiency, retinal vein occlusion, legg-calve-perthes disease, liver disease, hypertension, pulmonary embolism, lupus, and patent foramen ovale.

Long Name

Coagulation Factor V

Alternate Names

Cf-5, Cf5, Coagulation Factor 5, F5, Factor V, FVL, PCCF, Proaccelerin, Labile Factor, RPRGL1, THPH2

Gene Symbol

F5

Additional Coagulation Factor V Products

Product Documents for Human Factor V ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Factor V ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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