Rabbit FGF basic/FGF2/bFGF ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69962

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

View all FGF basic/FGF2/bFGF ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rabbit

Specificity

This kit recognizes Rabbit bFGF/FGF2 in samples. No significant cross-reactivity or interference between Rabbit bFGF/FGF2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.28%

Inter-Assay Precision (Precision between assays) CV% < 4.75%

Recovery for Rabbit FGF basic/FGF2/bFGF ELISA Kit (Colorimetric)

Recovery

88-106%

Linearity

Rabbit FGF basic/FGF2/bFGF ELISA Kit (Colorimetric)

Scientific Data Images for Rabbit FGF basic/FGF2/bFGF ELISA Kit (Colorimetric)

Rabbit FGF basic/FGF2/bFGF ELISA Kit (Colorimetric)

ELISA: Rabbit FGF basic/FGF2/bFGF ELISA Kit (Colorimetric) [NBP2-69962] -

ELISA: Rabbit FGF basic/FGF2/bFGF ELISA Kit (Colorimetric) [NBP2-69962] - Standard Curve Reference

Kit Contents for Rabbit FGF basic/FGF2/bFGF ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: FGF basic/FGF2/bFGF

FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Gene Symbol

FGF2

Additional FGF basic/FGF2/bFGF Products

Product Documents for Rabbit FGF basic/FGF2/bFGF ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rabbit FGF basic/FGF2/bFGF ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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