Sheep FSH ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60023

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

View all FSH ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Sheep

Specificity

This assay has high sensitivity and excellent specificity for detection of sheep FSH. No significant cross-reactivity or interference between sheep FSH and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 15

Inter-Assay Precision (Precision between assays) %CV < 15

Scientific Data Images for Sheep FSH ELISA Kit (Colorimetric)

ELISA: Sheep FSH ELISA Kit (Colorimetric) [NBP2-60023]

ELISA: Sheep FSH ELISA Kit (Colorimetric) [NBP2-60023]

ELISA: Sheep FSH ELISA Kit (Colorimetric) [NBP2-60023] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Sheep FSH ELISA Kit (Colorimetric)

Sheep FSH ELISA Kit (Colorimetric) [NBP2-60023] -

Sheep FSH ELISA Kit (Colorimetric) [NBP2-60023] - Standard Curve Reference

Kit Contents for Sheep FSH ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Standard
  • Stop Solution
  • Substrate A
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: FSH

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for FSH. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Additional FSH Products

Product Documents for Sheep FSH ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Sheep FSH ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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