Mouse Gastrin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11785

Novus Biologicals, part of Bio-Techne
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NBP3-11785
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

View all Gastrin I ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Precision

Intra-Assay Precision (Precision within an assay) %CV < 6.81

Inter-Assay Precision (Precision between assays) %CV < 5.46

Recovery for Mouse Gastrin ELISA Kit (Colorimetric)

Recovery

91 - 109%

Linearity

Mouse Gastrin ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Gastrin ELISA Kit (Colorimetric)

Mouse Gastrin ELISA Kit (Colorimetric)

ELISA: Mouse Gastrin ELISA Kit (Colorimetric) [NBP3-11785] -

ELISA: Mouse Gastrin ELISA Kit (Colorimetric) [NBP3-11785] - Standard Curve Reference

Kit Contents for Mouse Gastrin ELISA Kit (Colorimetric)

  • Biotinylated Detection Antibody Diluent
  • Concentrated Biotinylated Detection Antibody
  • Concentrated HRP Conjugate
  • Concentrated Wash Buffer
  • HRP Conjugate Diluent
  • Micro ELISA Plate
  • Plate Sealer
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Gastrin I

Gastrin, which is normally formed by mucosal cells in the gastric antrum and by the D cells of the pancreatic islets, is a hormone whose main function is to stimulate secretion of HCl by the gastric mucosa. HCl, in turn, inhibits gastrin formation. Gastrin also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. Gastrin is regulated by epidermal growth factor in both mice and humans. Gastrin is excreted in excess by pancreatic tumors in the Zollinger- Ellison syndrome. Gastrin maps to human chromosome 17q-21. Gastrin- Releasing Peptide (GRP) stimulates the release of gastrin as well as other gastrointestinal hormones, in addition to acting as an autocrine growth factor for certain cell types. High levels of GRP are found in the human lung just after birth and levels decrease thereafter in parallel with the observed disease in a number of pulmonary neuroendocrine cells. GRP is known to promote lung tumorigenesis in model systems and, interestingly, is induced by retinoic acid. GRP is involved in several functions with the hypothalamus, and is thought to play a role in regulating pituitary hormone secretion. GRP maps to human chromosome 18q21.

Alternate Names

G17, GAST, Gastrin-17

Gene Symbol

GAST

Additional Gastrin I Products

Product Documents for Mouse Gastrin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse Gastrin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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