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Rat GATA-4 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00468

Novus Biologicals, part of Bio-Techne
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NBP3-00468

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat GATA-4 in samples. No significant cross-reactivity or interference between Rat GATA-4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.87%

Inter-Assay Precision (Precision between assays) 4.66%

Recovery for Rat GATA-4 ELISA Kit (Colorimetric)

Recovery

88 - 106%

Linearity

Rat GATA-4 ELISA Kit (Colorimetric)

Scientific Data Images for Rat GATA-4 ELISA Kit (Colorimetric)

Rat GATA-4 ELISA Kit (Colorimetric)

ELISA: Rat GATA-4 ELISA Kit (Colorimetric) [NBP3-00468] -

ELISA: Rat GATA-4 ELISA Kit (Colorimetric) [NBP3-00468] - Standard Curve Reference

Kit Contents for Rat GATA-4 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GATA-4

GATA4 is a 46 kDa member of the GATA family of zinc finger containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs.

Alternate Names

GATA4

Gene Symbol

GATA4

Additional GATA-4 Products

Product Documents for Rat GATA-4 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat GATA-4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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