GDF-8/Myostatin ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DGDF80
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Tissue Homogenates (20 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
5.32 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for GDF-8/Myostatin Quantikine ELISA Kit
The Quantikine GDF-8/Myostatin Immunoassay is a 4.5 hour solid phase ELISA designed to measure GDF-8 levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant GDF-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant GDF-8 accurately. Results obtained using natural GDF-8 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural GDF-8.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Multi-Species
Specificity
Natural and recombinant human, mouse, rat, canine, porcine, and equine GDF-8.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 171 | 583 | 1147 | 146 | 491 | 1058 |
| Standard Deviation | 9.20 | 14.8 | 20.9 | 8.80 | 17.7 | 32.3 |
| CV% | 5.4 | 2.5 | 1.8 | 6.0 | 3.6 | 3.1 |
Recovery for GDF-8/Myostatin Quantikine ELISA Kit
The recovery of GDF-8 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 110 | 100-118 |
| Serum and Plasma (n=12) | 91 | 80-117 |
| Tissue Homogenates (n=4) | 100 | 90-120 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of GDF-8 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for GDF-8/Myostatin Quantikine ELISA Kit
Multi-species GDF-8/Myostatin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: GDF-8/Myostatin
Long Name
Growth Differentiation Factor 8
Alternate Names
GDF8, MSLHP, MSTN, Myostatin
Gene Symbol
MSTN
Additional GDF-8/Myostatin Products
Product Documents for GDF-8/Myostatin Quantikine ELISA Kit
Product Specific Notices for GDF-8/Myostatin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.