Rat Ghrelin/Obestatin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82143

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (50 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

View all Ghrelin/Obestatin ELISA Kits »

Product Specifications

Assay Type

Competitive-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Obestatin in samples. No Significant cross-reactivity or interference between Rat Obestatin and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6%

Inter-Assay Precision (Precision between assays) CV% < 7%

Recovery for Rat Ghrelin/Obestatin ELISA Kit (Colorimetric)

Recovery

average = 92% for serum; average = 92% for EDTA plasma; average = 100% for cell culture media

Linearity

Rat Ghrelin/Obestatin ELISA Kit (Colorimetric)

Scientific Data Images for Rat Ghrelin/Obestatin ELISA Kit (Colorimetric)

Rat Ghrelin/Obestatin ELISA Kit (Colorimetric)

ELISA: Rat Ghrelin/Obestatin ELISA Kit (Colorimetric) [NBP2-82143] -

ELISA: Rat Ghrelin/Obestatin ELISA Kit (Colorimetric) [NBP2-82143] - Standard Curve Reference

Kit Contents for Rat Ghrelin/Obestatin ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Ghrelin/Obestatin

Ghrelin is a 28 residue pepetide expressed by stomach cells that is responsible for the hunger sensation. Expression is increased during times of fasting, and decreased after food consumption. Ghrelin is initially derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 and is involved in satiety and decreased food intake. Ghrelin antibodies are useful tools for lipid and metabolism research as well as neuroscience studies.

Alternate Names

appetite-regulating hormone, ghrelin, ghrelin/obestatin preprohormone, ghrelin/obestatin prepropeptide, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, MTLRPgrowth hormone secretagogue receptor ligand, obestatin, Protein M46

Gene Symbol

GHRL

Additional Ghrelin/Obestatin Products

Product Documents for Rat Ghrelin/Obestatin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Ghrelin/Obestatin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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