Rat Glut4 ELISA Kit (Colorimetric)

Name: Rat Glut4 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-82176

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82176

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

0.38 ng/mL

Assay Range

0.63 - 40 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat GLUT4 in samples. No significant cross-reactivity or interference between Rat GLUT4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6%

Inter-Assay Precision (Precision between assays) CV% < 5%

Recovery for Rat Glut4 ELISA Kit (Colorimetric)

Recovery

average = 96% for serum; average = 94% for EDTA plasma; average = 90% for cell culture media

Linearity

Scientific Data Images for Rat Glut4 ELISA Kit (Colorimetric)

ELISA: Rat Glut4 ELISA Kit (Colorimetric) [NBP2-82176] -

ELISA: Rat Glut4 ELISA Kit (Colorimetric) [NBP2-82176] - Standard Curve Reference

Kit Contents for Rat Glut4 ELISA Kit (Colorimetric)

Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Glut4

The stimulation of glucose uptake by insulin in muscle and adipose tissue requires translocation of the GLUT4 glucose transporter from intracellular storage sites to the cell surface. Activation of phosphatidylinositol-3-OH kinase (PI3K) is required for this trafficking event, but it is not sufficient to produce GLUT4 translocation. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

Alternate Names

SLC2A4

Gene Symbol