Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78850

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, or cell culture supernatant (100 uL)

Sensitivity

100 pg/mL

Assay Range

0.5 - 32 ng/mL

View all Glutathione Peroxidase 3/GPX3 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA (quantitative)

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

This Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric) is to be used for the in vitro quantitative determination of human Glutathione Peroxidase 3/GPX3 in serum, plasma and cell culture supernatant.

Precision

Intra-Assay Precision (Precision within an assay) Sample 1: CV = 5.04% (n=12) Sample 2: CV = 4.77% (n=12) Sample 3: CV = 5.03% (n=12) Sample 4: CV = 8.46% (n=12) Sample 5: CV = 8.35% (n=12) Sample 6: CV = 9.64% (n=12) Sample 7: CV = 4.21% (n=12) Sample 8: CV = 4.24% (n=12)

Inter-Assay Precision (Precision between assays) Sample 1: CV = 4.83% (n=8) Sample 2: CV = 4.77% (n=8) Sample 3: CV = 4.17% (n=8) Sample 4: CV = 4.09% (n=8) Sample 5: CV = 4.66% (n=8) Sample 6: CV = 1.12% (n=8) Sample 7: CV = 4.79% (n=8) Sample 8: CV = 5.04% (n=8)

Recovery for Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric)

Recovery

92%

Scientific Data Images for Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric)

ELISA: Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric) - Sample standard curve for Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric)

Kit Contents for Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric)

  • 1 bottle Stop Solution (STOP)
  • 1 bottle TMB Substrate Solution (TMB)
  • 1 plate coated with human GPX3 Antibody
  • 1 vial Detection Antibody (DET)
  • 1 vial HRP Labeled Streptavidin (lyophilized) (STREP-HEP)
  • 1 vial human GPX3 Standard (lyophilized) (STD)
  • 2 bottles ELISA Buffer 10X
  • 2 bottles Wash Buffer 10X
  • 2 plate sealers (plastic film)
  • 2 silica Gel Minibags

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Glutathione Peroxidase 3/GPX3

Glutathione Peroxidase 3 (GPX3) belongs to the glutathione peroxidase family. It protects cells and enzymes from oxidative damage by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide by glutathione. There is increasing evidence that GPX3 is a novel tumor suppressor and a therapeutic target for insulin resistance and diabetes mellitus.

GPX-3 antibodies are useful tools for research on cellular response to oxidative stress as well as cancer and diabetes studies.

Alternate Names

GPX-P, GPX3

Gene Symbol

GPX3

Additional Glutathione Peroxidase 3/GPX3 Products

Product Documents for Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Glutathione Peroxidase 3/GPX3 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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