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Human Glutathione S-Transferase pi 1/GSTP1 ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25938

Novus Biologicals, part of Bio-Techne
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NBP3-25938

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.28 ng/mL

Assay Range

0.47 - 30 ng/mL

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human Glutathione S-Transferase pi 1/GSTP1 in samples. No significant cross-reactivity or interference between Human Glutathione S-Transferase pi 1/GSTP1 and analogues was observed.

Description

Assay Time: 4.5h

Precision

Intra-Assay Precision (Precision within an assay) CV% < 11.49

Inter-Assay Precision (Precision between assays) CV% < 12.00

Recovery

Recovery

87 - 115%

Scientific Data Images

Human Glutathione S-Transferase pi 1/GSTP1 ELISA Kit (Chemiluminescence)

Human Glutathione S-Transferase pi 1/GSTP1 ELISA Kit (Chemiluminescence) [NBP3-25938] -

Human Glutathione S-Transferase pi 1/GSTP1 ELISA Kit (Chemiluminescence) [NBP3-25938] - Standard Curve Reference

Kit Contents

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Glutathione S-Transferase pi 1/GSTP1

GSTP1 (glutathione-S-transferase, pi 1), also called GST3/DFN7, which is located on chromosome 11q13 , is a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 act like a tumor suppressor gene, which when inactivated leads to tumor growth, and the -class glutathione S-transferase is commonly inactivated by somatic CpGisland hypermethylation in cancers of the prostate, liver, and breast. Methylation of regulatory sequences at the GSTP1 gene locus is found in the vast majority (>90%) of prostate carcinomas and is associated with transcriptional down-regulation.

Alternate Names

DFN7, FAEES3, Glutathione STransferase pi 1, GSTP1

Gene Symbol

GSTP1

Additional Glutathione S-Transferase pi 1/GSTP1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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