Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric)

Name: Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-82519

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82519

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Glycogen Phosphorylase BB/GPBB in samples. No Significant cross-reactivity or interference between Human Glycogen Phosphorylase BB/GPBB and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.55

Inter-Assay Precision (Precision between assays) CV% < 4.28

Recovery for Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric)

Recovery

85-106%

Linearity

Scientific Data Images for Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric)

ELISA: Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric) [NBP2-82519] -

ELISA: Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric) [NBP2-82519] - Standard Curve Reference

Kit Contents for Human Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Glycogen Phosphorylase BB/GPBB

GPBB is found predominantly in the brain. Glycogen phosphorylase is a key enzyme of glycogenolysis, catalyzing the rate determining step in glycogen degradation. The activity of this enzyme is allosterically activated by AMP and alosterically inhibited by ATP, ADP, and glucose 6 phosphate.

Alternate Names

PYGB

Gene Symbol