HBsAg ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0286

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Plasma, Serum (50 uL)

Sensitivity

0.2 ng/mL

View all HBsAg ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Description

Hepatitis B surface antigen ELISA Kit is a sandwich enzyme immunoassay for the measurement of hepatitis B surface antigen.

Kit Contents for HBsAg ELISA Kit (Colorimetric)

  • 2 N sulfuric acid
  • Anti-HBs Peroxidase Solution
  • Anti-HBs Plate
  • Conc. Washing Solution D (20X)
  • HB Negative Control
  • HBsAg Positive Control
  • TMB Substrate Solution A
  • TMB Substrate Solution B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HBsAg

Hepatitis B surface antigen ELISA Kit is a solid-phase enzyme immunoassay (ELISA= enzyme-linked immuno-sorbent assay) based on the "sandwich principle". The solid phase of the microtiter plate is made of polystyrene wells coated with mouse monoclonal antibodies specific for HBsAg; whereas guinea pig polyclonal antibody purified by affinity chromatography is used to prepare the anti-HBs peroxidase (horseradish) conjugate in the liquid-phase. When a serum or plasma specimen containing HBsAg is added to the anti-HBs antibody-coated wells together with the peroxidase conjugated anti-HBs antibody and incubated, an antibody-HBsAg-antibody-peroxidase complex will form on the wells. After washing the microtiter plate to remove unbound material, a solution of TMB substrate is added to the wells and incubated. A color develops in proportion to the amount of HBsAg bound to Anti-HBs. The peroxidase-TMB reaction is stopped by addition of sulfuric acid. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650 nm.

Alternate Names

HBsAg, HBV major surface antigen, HBV surface antigen, Hepatitis B Virus major surface antigen, Hepatitis B Virus Surface Antigen, Major surface antigen, protein S

Gene Symbol

S

Additional HBsAg Products

Product Documents for HBsAg ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HBsAg ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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