Human Hemoglobin A1 ELISA Kit (Colorimetric)

Name: Human Hemoglobin A1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-42533

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42533

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Product Specifications
Precision
Recovery
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Erythrocyte lysates, Cell lysates, Cell culture supernates and other biological fluids (100 uL)

Sensitivity

0.54 ng/mL

Assay Range

1.56 - 100 ng/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay time: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 12

Recovery for Human Hemoglobin A1 ELISA Kit (Colorimetric)

Recovery

79 - 95%

Kit Contents for Human Hemoglobin A1 ELISA Kit (Colorimetric)

Assay Diluent A
Assay Diluent B
Detection Reagent A
Detection Reagent B
Instruction manual
Plate sealer for 96 wells
Pre-coated ready to use 96-well strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Hemoglobin A1

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.

Alternate Names

alpha one globin, alpha-1 globin, alpha-1-globin, alpha-globin, CD31, hemoglobin alpha 1 globin chain, Hemoglobin alpha chain, hemoglobin alpha-1 chain, hemoglobin subunit alpha, hemoglobin, alpha 1, MGC126895, MGC126897

Gene Symbol

HBA1

Additional Hemoglobin A1 Products

Product Documents for Human Hemoglobin A1 ELISA Kit (Colorimetric)

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Human Hemoglobin A1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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