Rat Hexokinase 1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82213

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

56.25 pg/mL

Assay Range

31.25 - 2000 pg/mL

View all Hexokinase 1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Hexokinase 1 in samples. No Significant cross-reactivity or interference between Rat Hexokinase 1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 11%

Inter-Assay Precision (Precision between assays) CV% < 11%

Recovery for Rat Hexokinase 1 ELISA Kit (Colorimetric)

Recovery

average = 95% for serum; average = 97% for EDTA plasma; average = 98% for cell culture media

Linearity

Rat Hexokinase 1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Hexokinase 1 ELISA Kit (Colorimetric)

Rat Hexokinase 1 ELISA Kit (Colorimetric)

ELISA: Rat Hexokinase 1 ELISA Kit (Colorimetric) [NBP2-82213] -

ELISA: Rat Hexokinase 1 ELISA Kit (Colorimetric) [NBP2-82213] - Standard Curve Reference

Kit Contents for Rat Hexokinase 1 ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Hexokinase 1

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Alternate Names

HK1, HKI

Gene Symbol

HK1

Additional Hexokinase 1 Products

Product Documents for Rat Hexokinase 1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rat Hexokinase 1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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