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Human HMBS ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39927

Novus Biologicals, part of Bio-Techne
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NBP3-39927

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.27 ng/mL (example only; lot dependent)

Assay Range

0.781 - 50 ng/mL (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human HMBS ELISA Kit (Colorimetric)

Human HMBS ELISA Kit (Colorimetric)

ELISA: Human HMBS ELISA Kit (Colorimetric) [NBP3-39927]

ELISA: Human HMBS ELISA Kit (Colorimetric) [NBP3-39927] - Standard Curve Reference

Kit Contents for Human HMBS ELISA Kit (Colorimetric)

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HMBS

HMBS encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.

Alternate Names

hydroxymethylbilane synthasePre-uroporphyrinogen synthase, PBGDPBG-D, PORC, porphobilinogen deaminase, porphyria, acute; Chester type, UPSEC 2.5.1.61, uroporphyrinogen I synthase, uroporphyrinogen I synthetase

Gene Symbol

HMBS

Additional HMBS Products

Product Documents for Human HMBS ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human HMBS ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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