Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18029

Novus Biologicals, part of Bio-Techne
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NBP3-18029
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

37.5 pg/mL

Assay Range

62.5-4000 pg/mL

View all HO-1/HMOX1/HSP32 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.45

Inter-Assay Precision (Precision between assays) %CV < 6.00

Recovery for Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

Recovery

84-102%

Linearity

Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

ELISA: Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric) [NBP3-18029] -

ELISA: Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric) [NBP3-18029] - Standard Curve Reference

Kit Contents for Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HO-1/HMOX1/HSP32

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Long Name

Heme Oxygenase 1

Alternate Names

HMOX1, HO1, HSP32

Gene Symbol

HMOX1

Additional HO-1/HMOX1/HSP32 Products

Product Documents for Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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