Human ADAM12 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DAD120
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), Heparin Plasma (25 µL), Urine (50 µL)
Sensitivity
0.076 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Urine)
Product Summary for Human ADAM12 Quantikine ELISA Kit
The Quantikine Human ADAM12 immunoassay is a 4.5 hour solid phase ELISA designed to measure human ADAM12 in cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human ADAM12 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural ADAM12 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring ADAM12.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human ADAM12. This kit recognizes pro-ADAM12, mature ADAM12, and ADAM12/TIMP-3 complex.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.39 | 3.57 | 6.84 | 1.43 | 3.62 | 6.74 |
| Standard Deviation | 0.057 | 0.131 | 0.235 | 0.079 | 0.145 | 0.299 |
| CV% | 4.1 | 3.7 | 3.4 | 5.5 | 4.0 | 4.4 |
Recovery for Human ADAM12 Quantikine ELISA Kit
The recovery of human ADAM12 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 98 | 88-105 |
| Heparin Plasma (n=4) | 97 | 92-106 |
| Serum (n=4) | 98 | 89-106 |
| Urine (n=4) | 99 | 91-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ADAM12 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human ADAM12 Quantikine ELISA Kit
Human ADAM12 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ADAM12
Long Name
A Disintegrin and Metalloprotease-like Domain 12
Alternate Names
Meltrin alpha
Entrez Gene IDs
8038 (Human)
Gene Symbol
ADAM12
Additional ADAM12 Products
Product Documents for Human ADAM12 Quantikine ELISA Kit
Product Specific Notices for Human ADAM12 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.