Human CCL24/Eotaxin-2/MPIF-2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCC240B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
14.3 pg/mL
Assay Range
78-5000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human CCL24/Eotaxin-2/MPIF-2 Quantikine ELISA Kit
The Quantikine Human Eotaxin-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Eotaxin-2 levels in cell culture supernates, serum, and plasma. It contains E.coli-expressed recombinant human Eotaxin-2 and antibodies raised against the recombinant factor. Results obtained for naturally occurring human Eotaxin-2 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Eotaxin-2.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Eotaxin-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 453 | 1239 | 2487 | 523 | 1379 | 2528 |
| Standard Deviation | 43 | 100 | 188 | 51.4 | 74.6 | 141 |
| CV% | 9.6 | 8.0 | 7.6 | 9.8 | 5.4 | 5.6 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 646 | 1856 | 3693 | 750 | 1971 | 3506 |
| Standard Deviation | 46 | 121 | 201 | 63.2 | 112 | 284 |
| CV% | 7.1 | 6.5 | 5.4 | 8.4 | 5.7 | 8.1 |
Recovery for Human CCL24/Eotaxin-2/MPIF-2 Quantikine ELISA Kit
The recovery of Eotaxin-2 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 78 | 72-84 |
| EDTA Plasma (n=4) | 104 | 82-117 |
| Heparin Plasma (n=4) | 99 | 80-115 |
| Serum (n=4) | 103 | 84-116 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of Eotaxin-2 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CCL24/Eotaxin-2/MPIF-2 Quantikine ELISA Kit
Human CCL24/Eotaxin-2/MPIF-2 ELISA Cell Culture Supernate Standard Curve
Human CCL24/Eotaxin-2/MPIF-2 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL24/Eotaxin-2/MPIF-2
Eotaxin-2, also named myeloid progenitor inhibitory factor 2 (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. Mouse Eotaxin-2 precursor protein shares approximately 58% amino acid sequence identity with human Eotaxin-2.
Alternate Names
Eotaxin-2, MPIF-2, MPIF2
Gene Symbol
CCL24
Additional CCL24/Eotaxin-2/MPIF-2 Products
Product Documents for Human CCL24/Eotaxin-2/MPIF-2 Quantikine ELISA Kit
Product Specific Notices for Human CCL24/Eotaxin-2/MPIF-2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.