Human CCL27/CTACK Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCC270
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
4.68 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human CCL27/CTACK Quantikine ELISA Kit
The Quantikine Human CTACK Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CTACK in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human CTACK and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CTACK showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring CTACK.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human CTACK
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 127 | 241 | 559 | 129 | 242 | 557 |
| Standard Deviation | 4.79 | 9.05 | 28.4 | 16.1 | 21.5 | 33.8 |
| CV% | 3.8 | 3.7 | 5.1 | 12.5 | 8.9 | 6.1 |
Recovery for Human CCL27/CTACK Quantikine ELISA Kit
The recovery of CTACK spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 91-105 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CTACK were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CCL27/CTACK Quantikine ELISA Kit
Human CCL27/CTACK ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL27/CTACK
CCL27, also known as Cutaneous T cell-attracting chemokine (CTACK), ALP, ILC and ESkine, is a CC chemokine discovered using expressed sequence tags (EST) in a homology search for novel chemokines. Mature human and mouse CCL27 are 84% similar. Mouse CCL27 shares 37% and 35% amino acid identity with mouse MIP-3 beta and mouse TECK, respectively. CCL27 is predominantly expressed in the skin. The expression is up-regulated by proinflammatory cytokines.
Alternate Names
CTACK, ILC
Gene Symbol
CCL27
Additional CCL27/CTACK Products
Product Documents for Human CCL27/CTACK Quantikine ELISA Kit
Product Specific Notices for Human CCL27/CTACK Quantikine ELISA Kit
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.