Human CDX2 DuoSet ELISA

Key Product Details

Product Specifications

Kit Contents for Human CDX2 DuoSet ELISA

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween^® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Also required:
Sample Diluent: IC Diluent #4 (1 mM EDTA, 0.5% TritonTM X-100 in PBS, pH 7.2-7.4, Catalog # DYC001).

Preparation and Storage

Background: CDX2

The caudal-type homeobox protein family of transcription factors are involved in early anteroposterior patterning by regulating members of the HOX gene family. CDX proteins contain a caudal-like activation domain and a DNA-binding homeodomain. CDX2 promotes intestinal patterning and development, while CDX4 regulates hematopoiesis and proliferation and differentiation of epithelial lining in the gut. Caudal homologs are associated with various diseases including cancers of the gut epithelium.

Additional CDX2 Products

• All Products for CDX2
• CDX2 ELISA Kits
• CDX2 Lysates
• CDX2 Primary Antibodies
• CDX2 Proteins and Enzymes