Human CEACAM-6/CD66c DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY3934
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
0.312-20 ng/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human CEACAM-6/CD66c DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human CEACAM-6/CD66c DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CEACAM-6. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human CEACAM-6/CD66c DuoSet ELISA
Human CEACAM-6 / CD66c ELISA Standard Curve
Kit Contents for Human CEACAM-6/CD66c DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CEACAM-6/CD66c
CEACAM-6, previously called nonspecific crossreacting antigen (NCA) or CD66c, is a highly glycosylated 90 kDa, 286 aa, GPI-linked membrane protein of the human CEACAM family within the immunoglobulin superfamily. CEACAM-6 is an intercellular adhesion molecule, forming homotypic and heterotypic bonds with CEACAM-1, -5 and -8. Mature human CEACAM-6 shares 84% and 85% aa identity with human CEACAMs 1 and 5 (CEA). It is expressed in azurophilic granules in cells of the granulocyte lineage, and in epithelia of various organs.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 6
Alternate Names
CD66c, CEACAM6, CEAL, NCA
Gene Symbol
CEACAM6
Additional CEACAM-6/CD66c Products
Product Documents for Human CEACAM-6/CD66c DuoSet ELISA
Product Specific Notices for Human CEACAM-6/CD66c DuoSet ELISA
For research use only
Loading...
Loading...
Loading...