Human IL-1 RII Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DR1B00
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)
Sensitivity
10 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human IL-1 RII Quantikine ELISA Kit
The Quantikine Human IL-1 sRII Immunoassay is a 3.5 hour solid phase ELISA designed to measure IL-1 sRII in serum, plasma, and cell culture supernates. It contains insect cell-expressed recombinant human IL-1 sRII and has been shown to quantitate the recombinant factor accurately. Results obtained using natural IL-1 sRII showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 sRII. The presence of high levels of IL-1 beta (> 7.5 ng/mL) in samples has been found to produce some interference (> 10% change) when assaying samples containing IL-1 sRII in the mid-level range.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-1 sRII
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 72.6 | 371 | 721 | 76.1 | 371 | 724 |
| Standard Deviation | 2.5 | 7.4 | 16.9 | 4.5 | 17.3 | 28.5 |
| CV% | 3.4 | 2.0 | 2.3 | 5.9 | 4.7 | 3.9 |
Recovery for Human IL-1 RII Quantikine ELISA Kit
The recovery of IL-1 sRII spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 95 | 86-102 |
| Citrate Plasma (n=5) | 101 | 92-109 |
| EDTA Plasma (n=5) | 101 | 95-107 |
| Heparin Plasma (n=5) | 100 | 91-110 |
| Serum (n=5) | 95 | 87-106 |
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-1 sRII in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-1 RII Quantikine ELISA Kit
Human IL-1 RII ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 RII
Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and, endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex.
IL-1 RII has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.
Long Name
Interleukin 1 Receptor II
Alternate Names
CD121b, IL-1 R beta, IL-1RII, IL1R2, IL1RII
Gene Symbol
IL1R2
Additional IL-1 RII Products
Product Documents for Human IL-1 RII Quantikine ELISA Kit
Product Specific Notices for Human IL-1 RII Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.