Human IL-4I1 DuoSet ELISA

Name: Human IL-4I1 DuoSet ELISA
Brand: R&D Systems
SKU: DY5684-05

R&D Systems, part of Bio-Techne | Catalog # DY5684-05

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Product Summary
Product Specifications
Kit Contents
Other Reagents Required
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

125-8000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human IL-4I1 DuoSet ELISA Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

View all IL-4I1 ELISA Kits »

Product Summary for Human IL-4I1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Interleukin 4 induced protein 1 (IL-4I1). The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Kit Contents for Human IL-4I1 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 3 (5 plates):(Catalog #DY009) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 3.

Normal Goat Serum:(Catalog #DY005)

The components listed above may be purchased separately:

PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer:(Catalog #WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent:(Catalog #DY004), or 5% Tween 20 in PBS, 7.2-7.4, 0.2 μm filtered

Substrate Solution:1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog #DY999)

Stop Solution:2 N H₂SO₄(Catalog #DY994)

Microplates: R&D Systems (Catalog #DY990)

Plate Sealers:ELISA Plate Sealers (Catalog #DY992)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-4I1

Interleukin 4 induced protein 1 (IL-4I1), also known as protein FIG-1 and L-amino acid oxidase, is encoded by a B-cell IL-4-inducible gene, FIG1, and is highly expressed in primary metastinal B-cell lymphomas. It belongs to the flavin monoamine oxidase family, FIG1 subfamily. Enzymological characterization reveals that IL-4I1 has L-amino acid oxidase activity with preference toward aromatic amino acids. Studies have shown that hIL-4I1 inhibited the proliferation of CD3-stimulated T lymphocytes with a similar effect on CD4(+) and CD8(+) T cells. Its inhibitory effect was dependent on enzymatic activity and H₂O₂ production. Its restricted expression to lymphoid tissues indicates that it may play an important function in the immune system.

Long Name

Interleukin 4-induced Protein-1

Alternate Names

FIG1, IL4I1

Entrez Gene IDs

259307 (Human); 14204 (Mouse); 308575 (Rat); 102115096 (Cynomolgus Monkey)

Gene Symbol

IL4I1

Additional IL-4I1 Products

Product Documents for Human IL-4I1 DuoSet ELISA

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human IL-4I1 DuoSet ELISA

For research use only

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