Human LIF Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DLF00
Key Product Details
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL)
Sensitivity
8 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human LIF Quantikine ELISA Kit
The Quantikine Human LIF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure soluble human LIF in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human LIF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant LIF accurately. Results obtained measuring natural human LIF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human LIF.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human LIF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 341 | 875 | 1358 | 81.6 | 472 | 910 |
| Standard Deviation | 12.3 | 34.5 | 42.5 | 4.4 | 17.7 | 35.2 |
| CV% | 3.6 | 3.9 | 3.1 | 5.4 | 3.8 | 3.9 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 314 | 868 | 1347 | 87.4 | 524 | 1017 |
| Standard Deviation | 7.7 | 24.3 | 23.8 | 6.2 | 34.9 | 46.5 |
| CV% | 2.5 | 2.8 | 1.8 | 7.1 | 6.7 | 4.6 |
Recovery for Human LIF Quantikine ELISA Kit
The recovery of LIF spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 91-106 |
| EDTA Plasma (n=2) | 89 | 84-97 |
| Heparin Plasma (n=8) | 92 | 84-100 |
| Serum (n=11) | 102 | 91-110 |
Linearity
Scientific Data Images for Human LIF Quantikine ELISA Kit
Human LIF ELISA Cell Culture Supernate Standard Curve
Human LIF ELISA Standard Curve
Human LIF ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: LIF
Long Name
Leukemia Inhibitory Factor
Alternate Names
D Factor, Emfilermin, HILDA, MLPLI
Gene Symbol
LIF
Additional LIF Products
Product Documents for Human LIF Quantikine ELISA Kit
Product Specific Notices for Human LIF Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.