Human MFG-E8 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DFGE80
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL), Saliva (50 µL), Urine (50 µL), Human Milk (10 µL)
Sensitivity
13.4 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Product Summary for Human MFG-E8 Quantikine ELISA Kit
The Quantikine Human MFG-E8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MFG-E8 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human MFG-E8 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MFG-E8. Results obtained using natural human MFG-E8 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MFG-E8.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human MFG-E8.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Human Milk, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 680 | 1181 | 2103 | 692 | 1262 | 2206 |
| Standard Deviation | 30.5 | 38.9 | 84.7 | 57.6 | 88.0 | 125 |
| CV% | 4.5 | 3.3 | 4.0 | 8.3 | 7.0 | 5.7 |
EDTA Plasma, Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 645 | 1143 | 2168 | 749 | 1326 | 2339 |
| Standard Deviation | 39.5 | 46.6 | 81.1 | 57.4 | 78.0 | 117 |
| CV% | 6.1 | 4.1 | 3.7 | 7.7 | 5.9 | 5.0 |
Recovery for Human MFG-E8 Quantikine ELISA Kit
The recovery of human MFG-E8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 100-110 |
| EDTA Plasma (n=4) | 96 | 89-102 |
| Heparin Plasma (n=4) | 96 | 89-102 |
| Serum (n=4) | 101 | 92-110 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of MFG-E8 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Human MFG-E8 Quantikine ELISA Kit
Human MFG-E8 ELISA Serum/Plasma/Saliva Standard Curve
Human MFG-E8 ELISA Cell Culture Supernate/Urine/Human Milk Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MFG-E8
Long Name
Milk Fat Globule EGF Factor 8
Alternate Names
BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, MFGE8, SED1
Gene Symbol
MFGE8
Additional MFG-E8 Products
Product Documents for Human MFG-E8 Quantikine ELISA Kit
Product Specific Notices for Human MFG-E8 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.