Human Nectin-4 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DNEC40
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (25 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (13 µL)
Sensitivity
16.6 pg/mL
Assay Range
39.1-2500 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human Nectin-4 Quantikine ELISA Kit
The Quantikine Human Nectin-4 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Nectin-4 in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant human Nectin-4 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Nectin-4 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Nectin-4.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Nectin-4.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 163 | 425 | 1365 | 194 | 474 | 1440 |
| Standard Deviation | 5.56 | 13.9 | 32.7 | 15.8 | 27.9 | 65.8 |
| CV% | 3.4 | 3.3 | 2.4 | 8.1 | 5.9 | 4.6 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 232 | 603 | 1867 | 245 | 595 | 1801 |
| Standard Deviation | 7.19 | 37.7 | 40.1 | 18.9 | 31.9 | 82.3 |
| CV% | 3.1 | 6.3 | 2.1 | 7.7 | 5.4 | 4.6 |
Recovery for Human Nectin-4 Quantikine ELISA Kit
The recovery of human Nectin-4 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 94-110 |
| EDTA Plasma (n=4) | 89 | 80-109 |
| Heparin Plasma (n=4) | 91 | 82-99 |
| Serum (n=4) | 90 | 80-101 |
| Urine (n=4) | 100 | 93-108 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Nectin-4 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Nectin-4 Quantikine ELISA Kit
Human Nectin-4 ELISA Serum/Plasma Standard Curve
Human Nectin-4 ELISA Cell Culture Supernate/Cell Lysate/Urine Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Nectin-4
Nectins are type I transmembrane glycoproteins that are calcium-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs). Homology has led to their designation as poliovirus receptor-related (PRR) proteins, but nectins do not bind poliovirus. The extracellular domains of the nectin family form cis-homodimers (same cell), followed by homophilic or heterophilic trans-dimers (across cells) that organize intercellular junctions. Nectin-1, also known as CD111, PRR1 and herpes virus entry mediator C (HVEC) has a secreted splice variant. Nectin-1, Nectin-2 (CD112, PRR2, HVEB) and Nectin-3 (PRR3) are concentrated in adherens junctions and exist on neurons, endothelial cells, epithelial cells and fibroblasts.
Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the placenta, the embryo, and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17.
Long Name
Poliovirus Receptor Related 4
Alternate Names
LNIR, Nectin4, PRR4, PVRL4
Gene Symbol
NECTIN4
Additional Nectin-4 Products
Product Documents for Human Nectin-4 Quantikine ELISA Kit
Product Specific Notices for Human Nectin-4 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.