Human Renin Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DREN00
This assay detects pro- and mature Renin.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (50 µL)
Sensitivity
14.8 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human Renin Quantikine ELISA Kit
The Quantikine Human Renin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human mature Renin and Prorenin in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human Renin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Renin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Renin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Renin. This assay recognizes both the Pro- and mature versions of recombinant human Renin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the
presence of human Renin in this assay. No medical histories were available for the donors used
in this study.
ND=Non-detectable
| Sample type | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=35) | 776 | 232-1892 | 374 |
| EDTA plasma (n=35) | 726 | 201-1852 | 365 |
| Heparin plasma | 680 | 179-1760 | 349 |
| Sample Type | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Urine (n=12) | 60.4 | 42 | ND-96.7 |
Cell Culture Supernates - CCD-1070Sk human foreskin fibroblasts were cultured in MEM
supplemented with 10% fetal bovine serum and 1 mM sodium pyruvate and grown for 8 days
until confluent. An aliquot of the cell culture supernate was removed, assayed for human
Renin, and measured 197 pg/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 201 | 599 | 1193 | 191 | 566 | 1153 |
| Standard Deviation | 10.6 | 14.2 | 19.9 | 10.5 | 27.1 | 46.4 |
| CV% | 5.3 | 2.4 | 1.7 | 5.5 | 4.8 | 4.0 |
Recovery for Human Renin Quantikine ELISA Kit
The recovery of Renin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 100 | 93-106 |
| EDTA Plasma (n=4) | 101 | 95-107 |
| Heparin Plasma (n=4) | 105 | 99-114 |
| Serum (n=4) | 101 | 90-109 |
| Urine (n=4) | 93 | 80-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Renin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Renin Quantikine ELISA Kit
Human Renin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Renin
Renin is a secreted endopeptidase that plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of Renin. The 406 aa precursor includes a signal peptide (aa 1 - 23), a propeptide (aa 24 - 66), and a mature chain (aa 67 - 406). The amino acid sequence of human Renin is 100%, 73%, 71% and 67% identical to that of chimpanzee, canine, mouse and rat.
Mouse Renin is a secreted, 42 - 47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin-1) is synthesized as a 381 aa proform (aa 22 - 402). In the kidney, prorenin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro-renin shares 70% and 85% aa sequence identity with human and rat pro-renin, respectively.
Additional Renin Products
Product Documents for Human Renin Quantikine ELISA Kit
Product Specific Notices for Human Renin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.