For the Human S100A8/S100A9 Heterodimer DuoSet ELISA, Catalog # DY8226-05, can Tween 20 be substituted for Brij® 35 in the Reagent Diluent?

Name: Human S100A8/S100A9 Heterodimer DuoSet ELISA
Brand: R&D Systems
SKU: DY8226-05
Rating: 5 (4 reviews)

The 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij® 35, pH 7.45-7.5 that we recommend as a reagent diluent for this kit is unique, which means that our lab would have found that more traditional reagent diluents do not perform optimally. It is certainly possible that you could potentially substitute a different detergent (such as Tween) with viable results, but the performance of this different formulation of the reagent diluent would need to be empirically determined on your end.

Human S100A8/S100A9 Heterodimer DuoSet ELISA

R&D Systems, part of Bio-Techne | Catalog # DY8226-05

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Product Summary
Product Specifications
Scientific Data
Kit Contents
Other Reagents Required
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

93.8-6000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human S100A8/S100A9 Heterodimer DuoSet ELISA Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

View all S100A8/S100A9 Heterodimer ELISA Kits »

Product Summary for Human S100A8/S100A9 Heterodimer DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant human S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9 (S100A8/S100A9 Heterodimer). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, plasma, saliva, human milk, urine, and fecal samples. Diluents for complex matrices, such as serum, plasma, saliva, human milk, urine, and feces, should be evaluated prior to use in this DuoSet.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human S100A8/S100A9 Heterodimer DuoSet ELISA

Human S100A8 / S100A9 Heterodimer ELISA Standard Curve

Kit Contents for Human S100A8/S100A9 Heterodimer DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: S100A8/S100A9 Heterodimer

S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca²⁺-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca²⁺-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Long Name

S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9

Alternate Names

Calprotectin

Entrez Gene IDs

6279 (Human)

Gene Symbol

S100A8

Additional S100A8/S100A9 Heterodimer Products

Product Documents for Human S100A8/S100A9 Heterodimer DuoSet ELISA

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Product Specific Notices for Human S100A8/S100A9 Heterodimer DuoSet ELISA

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