Human TFPI Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DTFP10
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL), Urine (50 µL)
Sensitivity
6.7 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Product Summary for Human TFPI Quantikine ELISA Kit
The Quantikine Human TFPI Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human TFPI in cell culture supernates, plasma, and urine. It contains NS0-expressed recombinant human TFPI and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TFPI showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TFPI.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human TFPI. This kit recognizes predominantly free TFPI and a very small percentage of the LDL- and HDL-bound TFPI.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 299 | 554 | 1108 | 301 | 569 | 1127 |
| Standard Deviation | 13.5 | 22.2 | 40.3 | 18.5 | 30.6 | 66.2 |
| CV% | 4.5 | 4.0 | 3.6 | 6.1 | 5.4 | 5.9 |
Recovery for Human TFPI Quantikine ELISA Kit
The recovery of TFPI spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 92 | 85-100 |
| Urine (n=4) | 94 | 85-106 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of TFPI were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human TFPI Quantikine ELISA Kit
Human TFPI ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TFPI
Long Name
Tissue Factor Pathway Inhibitor
Alternate Names
EPI, LACI
Gene Symbol
TFPI
Additional TFPI Products
Product Documents for Human TFPI Quantikine ELISA Kit
Product Specific Notices for Human TFPI Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.