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Rat IBSP/Sialoprotein II ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70022

Novus Biologicals, part of Bio-Techne
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NBP2-70022

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat BSP in samples. No significant cross-reactivity or interference between Rat BSP and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.15%

Inter-Assay Precision (Precision between assays) CV% < 5%

Recovery for Rat IBSP/Sialoprotein II ELISA Kit (Colorimetric)

Recovery

86-100%

Linearity

Rat IBSP/Sialoprotein II ELISA Kit (Colorimetric)

Scientific Data Images for Rat IBSP/Sialoprotein II ELISA Kit (Colorimetric)

Rat IBSP/Sialoprotein II ELISA Kit (Colorimetric)

ELISA: Rat IBSP/Sialoprotein II ELISA Kit (Colorimetric) [NBP2-70022] -

ELISA: Rat IBSP/Sialoprotein II ELISA Kit (Colorimetric) [NBP2-70022] - Standard Curve Reference

Kit Contents for Rat IBSP/Sialoprotein II ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IBSP/Sialoprotein II

The protein encoded by the Sialoprotein II gene is a major structural protein of the bone matrix. It constitutes approximately 12% ofthe noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophicchondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is thetrophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cellattachment through an RGD sequence that recognizes the vitronectin receptor. (provided by RefSeq)

Long Name

Integrin-binding Sialoprotein

Alternate Names

BNSP, BSP-II, SP-II

Gene Symbol

IBSP

Additional IBSP/Sialoprotein II Products

Product Documents for Rat IBSP/Sialoprotein II ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat IBSP/Sialoprotein II ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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