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Rat IgG ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11843

Novus Biologicals, part of Bio-Techne
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NBP3-11843

Key Product Details

Sample Type & Volume Required Per Well

serum, plasma and other biological fluids (100 uL)

Sensitivity

4.69 pg/mL

Assay Range

7.81 - 500 pg/mL

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Rat

Specificity

This kit recognizes Rat IgG in samples. No significant cross-reactivity or interference between Rat IgG and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10.06

Inter-Assay Precision (Precision between assays) %CV < 11.71

Recovery for Rat IgG ELISA Kit (Chemiluminescence)

Recovery

87 - 106%

Scientific Data Images for Rat IgG ELISA Kit (Chemiluminescence)

ELISA: Rat IgG ELISA Kit (Chemiluminescence) [NBP3-11843]

ELISA: Rat IgG ELISA Kit (Chemiluminescence) [NBP3-11843]

ELISA: Rat IgG ELISA Kit (Chemiluminescence) [NBP3-11843] - Representative standard curve.

Kit Contents for Rat IgG ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100X)
  • Concentrated HRP Conjugate (100X)
  • Concentrated Wash Buffer (25X)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IgG

IgG is a glycoprotein that is produced by plasma cells in response to an immunogen. It is the only class of immunoglobulins that can cross the placenta, which is mediated by a receptor on placental cells in the Fc region. It is the most versatile immunoglobulin because it is capable of carrying out all of the functions of immunoglobulin molecules. IgG fixes complement and binds to the Fc receptors on leukocytes, monocytes and macrophages; this causes the cell to internalize the antigen.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG, IP Detection Reagent

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Rat IgG ELISA Kit (Chemiluminescence)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat IgG ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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