Porcine IgM ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12525

Novus Biologicals, part of Bio-Techne
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NBP3-12525
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Colostrum, Milk

Assay Range

1.37 - 1000 ng/mL

View all IgM ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Porcine

Description

Detection method: Biotinylated-Streptavidin-HRP

Scientific Data Images for Porcine IgM ELISA Kit (Colorimetric)

ELISA: Porcine IgM ELISA Kit (Colorimetric) [NBP3-12525]

ELISA: Porcine IgM ELISA Kit (Colorimetric) [NBP3-12525]

ELISA: Porcine IgM ELISA Kit [NBP3-12525] - Typical standard curve.

Kit Contents for Porcine IgM ELISA Kit (Colorimetric)

  • Dilution Buffer B (10X) - 25 mL
  • HRP Solution A - 12 mL
  • Pig IgM Detection Antibody - 12 mL
  • Pig IgM Pre-Coated 96-well Strip Plate - 1 EA
  • Pig IgM Standard (1000 ng / vial) - 2 EA
  • Sealing Tape - 6 sheets
  • Stop Solution - 12 mL
  • TMB Substrate - 12 mL
  • Wash Buffer (20X) - 50 mL

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: IgM

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbol

IGHM

Additional IgM Products

Product Documents for Porcine IgM ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Porcine IgM ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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