Rat IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92699

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

4.0 pg/mL

Assay Range

15.6 - 1,000 pg/mL

View all IL-1 alpha/IL-1F1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-1 alpha positive serum. There was no crossreactivity detected

Description

The rat IL-1alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat IL-1alpha

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.0%

Inter-Assay Precision (Precision between assays) CV% < 10%

Scientific Data Images for Rat IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

ELISA: Rat IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric) [NBP1-92699]

ELISA: Rat IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric) [NBP1-92699]

ELISA: IL-1 alpha/IL-1F1 ELISA Kit [Biotin] [NBP1-92699] - Standard curve

Kit Contents for Rat IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to rat IL-1alpha
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-rat IL-1alpha polyclonal antibody
  • Rat IL-1alpha Standard lyophilized, 2 ng/ml upon reconstitution
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-1 alpha/IL-1F1

The interleukin-1 species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses. Two distinct IL-1 species, IL-1alpha and IL-1beta, have been identified. They share similarities such as the same molecular weight, similar biological effects and the same receptors on target cells. IL-1 proteins are produced by macrophages, monocytes and various other cell types such as adult T cell leukemias, fibroblasts, epithelial or endothelial cells, neutrophils and astrocytes. Their biological properties include pyrogenicity, bone resorption, presentation of antigen to T cells and stimulation of B and T lymphocyte proliferation.

Long Name

Interleukin 1 alpha

Alternate Names

BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM

Gene Symbol

IL1A

Additional IL-1 alpha/IL-1F1 Products

Product Documents for Rat IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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